Performance of a deep learning based neural network in the selection of human blastocysts for implantation 
Here, we evaluated the use of a deep convolutional neural network (CNN), trained using single timepoint images of embryos collected at 113 hr post-insemination, in embryo selection amongst 97 clinical patient cohorts (742 embryos) and observed an accuracy of 90% in choosing the highest quality embryo available.
Do human embryos have the ability of self-correction? 
Of the 9 euploid blastocysts, four showed euploid debris, while in the others, the debris were aneuploid. In the remaining pairs, the debris showed additional aneuploidy to those presented by their corresponding blastocyst. The observed ability of human embryos to self-correction doubts many invasive and non-invasive preimplantation testing for aneuploidy at the blastocyst stage, rendering high rate of false positive (discarding "good" embryos)
The duration of embryo culture after mouse IVF differentially affects cardiovascular and metabolic health in male offspring 
Using a mouse model, we demonstrate that in male but not female offspring, adverse cardiovascular (CV) health was more likely with prolonged culture to the blastocyst stage, but metabolic dysfunction was more likely if embryo transfer (ET) occurred at the early cleavage stage.
Pregnancy and Lactation in a 67-Year-Old Elderly Gravida following Donor Oocyte In Vitro Fertilization 
This case represents the eldest gravidae identified in the literature and illustrates the potential for a relatively uncomplicated perinatal course with successful lactation. This case may enable other providers to counsel elderly patients on anticipated outcomes inclusive of ability to breastfeed.
Characteristics of the IVF Cycle that Contribute to the Incidence of Mosaicism 
With the use of a proprietary algorithm for automated diagnosis of aneuploidy and mosaicism, we retrospectively analyzed a large series of 115,368 trophectoderm biopsies from 27,436 PGT-A cycles to determine whether certain biological factors and in vitro fertilization (IVF) practices influence the incidence of overall aneuploidy, whole uniform aneuploidy, mosaicism, and TE biopsies with only segmental aneuploidy
Normalized Ploidy Following 20 Consecutive Blastocysts with Chromosomal Error: Healthy 46, XY Pregnancy with IVF after Intraovarian Injection of Autol 
It appears that intraovarian application of autologous platelet-derived growth factors, when used before IVF, can impact oocyte integrity and facilitate euploid blastocyst development. Although research on intraovarian injection of autologous activated platelet rich plasma has already shown improved quantitative IVF responses, this is the first description of qualitative improvements in embryo genetics after intraovarian injection of autologous p
Mechanisms of human embryo development: from cell fate to tissue shape and back 
Here, I discuss the evidence that supports a role for the crosstalk between cell fate and tissue shape during early human embryogenesis. This is a critical developmental period, when the body plan is laid out and many pregnancies fail
Correlation between aneuploidy, standard morphology evaluation and morphokinetic development in 1730 biopsied blastocysts: a consecutive case series s 
Correlations were observed, in that euploid human blastocysts showed a higher percentage with top quality inner cell mass (ICM) and trophectoderm (TE), higher expansion grades and shorter time to start of blastulation, expansion and hatching, compared to aneuploid ones.
Women could conceive after ovarian tumors, study shows 
Women receiving fertility-sparing surgery for treatment of borderline ovarian tumours were able to have children, a study from Karolinska Institutet in Sweden published in Fertility & Sterility shows. Natural fertility was preserved in most of them and only a small proportion required assisted reproductive treatment such as in vitro fertilization. Survival in the group was also as high as in women who had undergone radical surgical for treatment
Noninvasive preimplantation genetic testing for aneuploidies (niPGT-A) and the principle of primum non nocere 
Perhaps with the intention of obtaining larger amounts of free-DNA, some groups are routinely postponing and establishing free-DNA collection in culture medium for Noninvasive preimplantation genetic testing for aneuploidies (niPGT-A) to day 6 for all blastocysts.
We have trained ERICA to rank embryos based on ploidy and implantation potential using single static embryo image. This tool represents a potentially significant advantage to assist embryologists to choose the best embryo, saving time spent annotating and does not require time lapse or invasive biopsy
Failure mode and effects analysis of witnessing protocols for ensuring traceability during PGD/PGS cycles 
Specific corrective measures were then introduced and implemented, and a second evaluation performed six months later. The meticulous and careful application of such measures allowed the risks to be decreased along the whole protocol, by reducing their estimated occurrence and/or increasing detection possibilities.
Preimplantation genetic testing as a component of root cause analysis of errors and reassignment of embryos in IVF 
The risks of embryo/gamete mix-up are a threat to the integrity of the IVF process, with significant implications for affected families. The use of preimplantation genetic testing through single-nucleotide polymorphism array or next-generation sequencing technology can help to identify, characterize and ultimately help, in some cases, to find the root cause, and to mitigate the extent of these errors for a given patient or laboratory.
Blastocyst contractions are strongly related with aneuploidy, lower implantation rates, and slow-cleaving embryos: a time lapse study 
Two of the traits for which the embryos included in this study were compared were statistically different. Embryos in the CT group had lower implantation rates, took longer to reach the blastocyst stage, and had a higher chance of being aneuploid, regardless of maternal age. Therefore, embryo contraction might be a useful parameter in the selection of embryos for transfer.
Developmental potential of aneuploid human embryos cultured beyond implantation 
We show that while trisomy 15 and trisomy 21 embryos develop similarly to euploid embryos, monosomy 21 embryos exhibit high rates of developmental arrest, and trisomy 16 embryos display a hypo-proliferation of the trophoblast, the tissue that forms the placenta.
Clinical Outcomes of Single Mosaic Embryo Transfer: High-Level or Low-Level Mosaic Embryo, Does it Matter? 
Comparable live birth rate (LBR) but higher miscarriage rate (MR) was found in the high-level group. (LBR: low vs. high: 44.5% vs. 36%, p=0.45, MR: low vs. high: 5.1% vs. 30.7%, p=0.012). Analyses of TE and ICM from the remaining mosaic blastocysts show a poor concordance.
Minimizing mosaicism: assessing the impact of fertilization method on rate of mosaicism after next-generation sequencing (NGS) preimplantation genetic 
IVF and ICSI NGS PGT-A have similar rates of euploid, aneuploid, and no result embryos, though IVF may result in higher rates of mosaicism and demonstrates differences in proportions of mosaic and aneuploid subtypes compared to ICSI. ICSI may be preferable to conventional insemination to minimize the rate of mosaic results in NGS PGT-A cycles
One hundred mosaic embryos transferred prospectively in a single clinic: exploring when and why they result in healthy pregnancies 
After euploid embryos, mosaic embryos can be considered for transfer, prioritizing those of the single segmental mosaic type. If a patient has mosaic embryos available that were generated at different ages, preference should be given to those made at younger ages.
The birth of a baby with mosaicism resulting from a known mosaic embryo transfer: a case report 
This is the first reported case of true fetal mosaicism resulting in a live birth following the transfer of a known mosaic embryo. Worldwide, prenatal diagnosis has shown the depletion of mosaicism in embryos transferred after they have been reported as mosaics. Our case demonstrates the need for close prenatal monitoring and diagnosis by early amniocentesis, preferably at >14 weeks gestation.
The combined effect of obesity and aging on human sperm DNA methylation signatures: inclusion of BMI in the paternal germ line age prediction model 
However, it is important to note that within each age category, high BMI individuals were predicted to be older on average than their actual age (about 1.4 years), which was not observed in the normal BMI group.
Degenerated oocyte in the cohort adversely affects IVF outcome 
Fertilization rate, pregnancy and clinical pregnancy rates were comparable between the two groups, however, the morphokinetics and developmental scores of the embryos were significantly worse in the DEG group.
The reproducibility of trophectoderm biopsies in euploid, aneuploid, and mosaic embryos using independently verified next-generation sequencing (NGS): 
TE biopsies predict euploidy or aneuploidy in the ICM with a high degree of accuracy. PGT-A with NGS of TE biopsies is shown to be highly reliable, with clinically relevant concordance rates for aneuploidy and euploidy over 95%.
Initial serum HCG levels are higher in pregnant women with a male fetus after fresh or frozen single blastocyst transfer: A retrospective cohort study 
Conclusions: MsHCG levels are higher in pregnant women with a male fetus than those with a female one on day 11 after fresh or frozen SBT. A sex-specific response to the stress in the process of in vitro embryo culture was suggested.
hcg/gender/gender and placental histopathology/maternal hcg and fetal gender/gender ration after sbt/maternal hcg and fresh x frozen sbt/
Measuring IGF-1 and IGFBP-3 Profiles in Women Seeking Assisted Reproduction: Relationship to Clinical Parameters (Study 1) 
The study shows a significant linear reduction in IGF-1 levels across the four age groups (<35 years, 35-39 years, 40-44 years and ≥:45 years: p < 0.001). However, there was no variation in IGFBP-3 levels but the IGF Ratio showed a progressive linear elevation with advancing age (p < 0.001). With respect to both BMI and stature, none of the IGF profile parameters showed any variation.
Exposure of human fallopian tube epithelium to elevated testosterone re 
How does exposure to a testosterone rich environment affect the function and gene expression of human fallopian tube epithelium (hFTE)? SUMMARY ANSWER. Elevated testosterone level alters several gene transcripts that regulate cilia expression and negatively impacts the rate of cilia beating.
Fallopian tube epithelial cells express androgen receptor and have a distinct hormonal responsiveness when compared with endometrial epithelium 
Proliferative indices, hormone receptor expression-scores and in vitro response to oestrogen and androgens of the human fallopian tube (FT) epithelium demonstrate a distinct pattern from the matched endometrium.
Preimplantation genetic testing for aneuploidies (abnormal number of chromosomes) in in vitro fertilisation 
There is insufficient good‐:quality evidence of a difference in cumulative live birth rate, live birth rate after the first embryo transfer, or miscarriage rate between IVF with and IVF without PGT‐:A as currently performed. No data were available on ongoing pregnancy rates. The effect of PGT‐:A on clinical pregnancy rate is uncertain.
Meanwhile, despite the lack of clear and convincing evidence to date, I will continue to test my couples with infertility for vitamin D deficiency, prescribe supplementation where needed, and monitor serum levels to ensure repletion
vitamin d/vitamin d and ivf outcome review & comment/
Clinical manifestations, risk factors, and maternal and perinatal outcomes of coronavirus disease 2019 in pregnancy: living systematic review and meta 
Pregnant and recently pregnant women are less likely to manifest covid-19 related symptoms of fever and myalgia than non-pregnant women of reproductive age and are potentially more likely to need intensive care treatment for covid-19. Pre-existing comorbidities,
Cycle day 2 insulin-like growth factor-1 serum levels as a prognostic tool to predict controlled ovarian hyperstimulation outcomes in poor responders 
Patients who respond poorly to controlled ovarian stimulation, despite normal cycle day 2 follicle-stimulating hormone levels, have significantly higher serum cycle day 2 IGF-1 levels when compared with age-matched normal and high responders.
ifg-1/poor ovarian response/day 2 igf-1 and poor responders/
In search of a new biomarker to predict poor ovarian response 
Optimizing outcomes for poor ovarian responders remains a challenge in the field of assisted reproductive technologies. A variety of stimulation protocols and adjunct medications have been developed and studied, with no clear advantage in this population
Although blastocyst-stage preimplantation genetic testing for aneuploidy (PGT-A) has been shown to improve implantation rates and enables near-universal single embryo transfer, it still requires an invasive biopsy and cryopreservation to allow time for genetic analysis
Does ICSI for in vitro fertilization cause more aneuploid embryos? 
These results indicate that more embryos produced by ICSI are aneuploidy as compared with embryos produced by regular IVF. The results indicate that in vitro manipulation of oocytes for ICSI procedure may have adverse effect on human oocytes, and it may be one of the reasons causing aneuploid embryos in human IVF.
Peripheral action of kisspeptin at reproductive tissues—role in ovarian function and embryo implantation and relevance to assisted reproductive techno 
Kisspeptin (KISS1) is encoded by the KISS1 gene and was initially found to be a repressor of metastasis. Natural mutations in the KISS1 receptor gene (KISS1R) were subsequently shown be associated with idiopathic hypothalamic hypogonadism and impaired puberty.
A PMMA-Based Microfluidic Device for Human Sperm Evaluation and Screening on Swimming Capability and Swimming Persistence 
This device can achieve a one-step procedure of high-quality sperm selection and achieve the quantitative evaluation of sperm swimming capability and persistence. Sperm with good swimming capability and persistence may be more suitable for fertilization in a viscous environment. This microdevice and methods could be used to guide the evaluation of sperm motility and screening in the future.
The Pregnancy Outcome of Mosaic Embryo Transfer: A Prospective Multicenter Study and Meta-Analysis 
Our meta-analysis concluded that METs gave rise to pregnancies but were associated with a reduced ongoing/live birth rate and a higher miscarriage rate. All 37 MET live births were confirmed viable, among which 8 completed prenatal genetic testing with normal results. Longitudinal investigation on one MET pregnancy evidenced the aneuploidy depletion hypothesis. This is the first multi-center prospective study reporting a full MET pregnancy outcom
mosaic embryo transfer (met)/pgt-a/management of mosaic embryos/mosaic/management of mosaic embryos [from abstract]/
Empathetic application of machine learning may address appropriate utilization of ART 
This commentary discusses challenges and strategies to providing personalized ART prognostics based on machine learning, and presents a case study where appropriate use of such prognostics in ART centres is associated with a trend towards increased ART utilization.
Man versus machine in IVF-can artificial intelligence replace physicians? 
However, the next step in quest of the use of AI in day-to-day IVF decision making during ovarian stimulation appears to be the development of an algorithm trained with large amount of data and integrated evidence-based recommendations (when to trigger, when to cancel, when to change the dose and by how much, when to ask the patient to return for monitoring) and to see if this algorithm would come up with different set of recommendations for cycl
Post-copulatory genetic matchmaking: HLA-dependent effects of cervical mucus on human sperm function 
This indicates that sperm fertilization capability may be dependent on the compatibility between cervical mucus (female) and sperm (male). We also found that sperm viability was associated with partners' HLA dissimilarity, indicating that cervical mucus may selectively facilitate later gamete fusion between immunogenetically compatible partners.
Ambient air pollution exposed during preantral-antral follicle transition stage was sensitive to associate with clinical pregnancy for women receiving 
..women aged 20-29 years old were more susceptible in preantral-antral follicle transition stage. Women aged 36-47 years old were more vulnerable during post-oocyte retrieve period. Our results suggested air pollution exposure during preantral-antral follicle transition stage was a note-worthy challenge to conceive among females receiving IVF.
air pollution/folliculogenesis/ambient air pollution/folliculogenesis and ivf/ambient air pollution, folliculogenesis and ivf/
Initial ovarian sensitivity index predicts embryo quality and pregnancy potential in the first days of controlled ovarian stimulation 
MOSI (modified ovarian sensitivity index) was highly correlated with key IVF parameters that are associated with achieved pregnancy. Using this index with antagonist cycles, clinicians may opt to stop an IVF cycle, under the assumption that the cycle will fail to produce good blastocysts, preventing wasting the patient’s resources and time.
PODCAST - Gestational Carriers: With Dr. James Goldfarb 
What’s the difference between a surrogate and a gestational carrier? Who needs a gestational carrier and how much does it cost? These questions and many more are discussed by interviewer, Dr. Brooke Rossi, with Dr. James Goldfarb.
MicroRNA expression profile analysis in sperm reveals hsa-mir-191 as an auspicious omen of in vitro fertilization 
These findings suggest that high hsa-mir-191-5p expression 9 [MicroRNAs (miRNAs)] in sperm is associated with early human embryonic quality and that hsa-mir-191-5p could be used as a potential marker to screen high-quality sperm to improve the success rates of in vitro fertilization (IVF).
Body mass index is not associated with donor oocyte recipient success: an ideal study using a paired analysis of sibling-oocytes 
In this idealized model that controls to the greatest degree possible for factors that would impact implantation, we found that a higher BMI did not reduce implantation, positive pregnancy, or delivery rates. These findings suggest that a higher BMI does not adversely affect uterine receptivity.